The ability of bacteria to form acid in some carbohydrate broths and not in others can be used in identification schemes. sanguinis SS910 are used as the positive and negative controls respectively. Quality controls, positive and negative reactions are determined each day the test is determined. Genprobe Accuprobe Pneumococcus Culture Identification Test, GEN-PROBE Inc. The AccuProbe- Pneumococcus test is used to aid in the identification of atypical pneumococci and to help differentiate between viridans Streptococcus strains. Too many colonies will result in a false positive test. Use care with the amount of colonies used. The test is performed according to the package insert instructions. Genprobe Accuprobe Enterococcus Culture Identification Test, GEN-PROBE Inc. The AccuProbe- Enterococcus test is used to aid in the identification of atypical enterococci and to help differentiate between Enterococcus and Lactococcus strains.Īn overnight culture grown on blood agar incubated 35☌ in CO 2. Vancomycin resistant streptococci or other unknowns other than leuconostocs and pediococci, which are intrinsically vancomycin resistant. If any of the results are unusual or not expected or the culture is contaminated the test must be repeated. The blood agar plates used in the manner described above are for checking for purity of cultures. Incubate the plate in CO 2 or a candle extinction jar at 35Ε C for 24 to 48 h.Įxamples of unusual or unexpected results: This test will determine if the unknown culture forms satellite colonies adjacent to the staphylococci, a characteristic of all NVS. Perpendicular to this streak, carefully make a single streak with a Staphylococcus aureus culture. If the culture is submitted as a nutritionally deficient Streptococcus (NVS), inoculate an entire trypticase soy blood agar plate with the culture.The 30 ml THB is used for serogrouping and serotyping in some cases. Some strains may take more than one day of incubation, no harm is done by incubating these broths longer than 24 to 72 h. Do not incubate longer than 18 h.For beta-hemolytic streptococci other than group A, a 30 ml THB can also be inoculated and placed at 35ΕC for 18 to 24 h. Place the 30 ml broth at 35ΕC for 16 to 18 h. The 5 ml THB is used for T-agglutination typing of group A streptococci. Place the 5 ml THB in a 30Ε C incubator or leave at room temperature for 1-3 days. For most cultures submitted as group A streptococci, those that appear pure on the submitted culture, a 30 ml and a 5 ml Todd-Hewitt broth (THB) should be inoculated. All plates should be incubated in a candle extinction jar or CO 2 incubator for 18 h at 35ΕC. If the culture is identified as a beta-hemolytic streptococcus, or group A, B, C, F, or G streptococci, inoculate a trypticase soy 5% sheep blood agar plate and place a bacitracin disk on the heavy part of the growth.Place a vancomycin disk on the heaviest part of the inoculum, and put the plate into a candle extinction jar or a CO 2 incubator for 18 to 24 h at 35C. Inoculate a trypticase soy 5% sheep blood agar plate by streaking a heavy inoculum onto one-fourth of the plate and streak the remaining portion for isolated colonies. If the culture is an unidentified gram-positive coccus, an Enterococcus, viridans Streptococcus, or of unknown identity (basically includes all cultures other than pneumococci, ß-hemolytic streptococci, and nutritionally variant streptococci), inoculate the following media.Outline of the Work Flow for the Streptococcus Laboratory
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